Science is inherently collaborative and context-embedded. It thrives on diversity, transparency, and education.

labSquared is an ongoing exploration of how academic research groups could function following these ideals. It is the handbook of an imaginary lab experimenting with ways of running itself: a laboratory laboratory.

I am not the first to think of many —perhaps any— of these proposals. But more importantly, the purpose is to build a repository of ideas and resources, a place to track, gather, and communicate these thoughts in. These can be starting points from which to trace courses in research, academic mentoring, science policy, science communication, and much more.

Understanding pathogen evolution

Understanding and predicting evolution can allow us to design drugs, vaccines, and public health policies that are more effective and robust. Achieving this requires new experimental, mathematical, and computational modeling tools.

Sequencing has revolutionized the study and management of infectious diseases. Despite the wealth of field data, we are missing tools with which to test hypotheses about genome evolution and epidemiology in a systematic manner. To address this, I have developed computational tools for simulating pathogen genetics and evolution in epidemiological models. I applied these tools to understand fundamental precepts in genomic epidemiology using small epidemiological simulations, in an independent collaboration with Prof. Vladimir Corredor and Dr. Mauricio Santos-Vega [article]. I look forward to expanding this work into a full research program by computationally predicting and experimentally validating intervention strategies that limit both the spread and the evolution of diseases.

South America has seen the worst increases in malaria cases of any continent over the last few years. The unique genetic makeup and slow-burning epidemiological dynamics of South American malaria raises questions about the evolutionary dynamics of the parasites it is caused by. As a member of an international work group of collaborators in Colombia, USA, and Europe, I help apply statistical methods to the genomic epidemiology of malaria along the Colombian Pacific coast [article].

The most common instance of resistance to treatment is antibiotic resistance in bacteria. Resistant bacteria in a biofilm may incur in fitness costs, and yet the benefits of resistance can often be shared among its neighbors. As a part of MTBI at Arizona State University, I co-developed a 3-D, spatially explicit agent-based model of bacterial resistance, to study spatial dynamics and competition within biofilms that can be harnessed to reduce resistance [technical report].

Phage therapy is an intriguing alternative to antibiotics, but is also subject to evolution of bacterial resistance. Although phage are capable of evolving too, this coevolution can be difficult to achieve. To make phage into effective therapeutic agents, we need ways of inhibiting bacterial evolution of resistance. At the CIMIC lab at Universidad de los Andes, I contributed to the development and validation of a dynamical model of phage therapy showing the potential of combining phage cocktails and antibiotics, even when the antibiotic is ineffective by itself [article].

Controlling expression and genetic circuits

Our ability to understand and control disease depends on our ability to control the genes underlying it. Synthetic biology allows us to repurpose systems with unrelated biological functions into tools for manipulating gene expression.

My Ph.D. research with the Niles Lab at MIT focuses on developing these kinds of tools for use in the malaria parasite, Plasmodium falciparum. I have assembled synthetic gene systems that allow robust, orthogonal control of transcription in malaria parasites, a task that had proven difficult to achieve thus far. I am now using these synthetic tools to characterize native transcriptional control mechanisms in the parasite, which can help discover new drug targets [in prep.].

Besides building tools to manipulate gene expression, it is important to understand how these tools function together. Genetic circuits exhibit context-dependent, emergent behaviors such as resource sharing, which complicate their use. In collaboration with the Del Vecchio lab at MIT, I helped assemble and model genetic devices to reduce these effects in model gene circuits [article].

Finally, I have built and modeled gene circuits for applications in new treatment strategies for bacterial disease, both as a member of Eligo Bioscience and in collaboration with the Collins lab at MIT [article, email me for pdf].

Probing and targeting pathogen genes

We can use engineered control of gene expression and bioinformatics to tease apart how pathogens live and die. This knowledge opens new paths to approach drug and vaccine design in a more informed manner.

Despite still killing more than 400,000 people a year, more than half of P. falciparum genes have unknown or hypothetical functions. To remedy this, I develop computational tools to visualize gene interactions and design gene editing for assaying gene function at a massive scale, as a part of the Niles Lab [article]. In addition, I use statistical approaches to interpret protein-compound interactions in order to establish potential drug targets and lead compounds [in prep.].

Another situation where we need a better understanding of gene function is bacterial persistence, the phenomenon in which bacteria stochastically enter a metabolic state that allows them to survive an antibiotic, despite being genetically susceptible. As a part of the Paulsson Lab at Harvard Medical School, I used microfluidics and live cell microscopy to screen gene function related to persistence in Escherichia coli.

Teaching Development Fellow – MIT Teaching and Learning Lab
(Fall 2022–Summer 2023)

Studied and developed practices to support graduate student teaching skills through observation and workshops. Developing workshops, programming, and written resources to support research project mentorship skills for graduate students throughout MIT. Completed courses on Subject Design, Lesson Planning, Microteaching, and Inclusive Teaching for a MIT TLL Teaching Certificate.

Data Fellow – MIT Biological Engineering Data Lab
(Spring 2020++)

Providing 1-on-1 coaching to undergraduates, graduate students, and postdocs at MIT for designing and executing projects in computational biology and biological data analysis. Offering language-specific training and support in Python, MATLAB, R, and cluster computing.

Peer counsellor – MIT Biological Engineering REFS
(Spring 2019–Summer 2023)

We provide 1-on-1 confidential conflict coaching, workshops, and reference material for navigating grad school to peer graduate students at the MIT Department of Biological Engineering. We identify points of stress in the Ph.D. track and work with the MIT BE Graduate Student Board to improve faculty mentorship and the graduate experience.

Teaching Assistant – MIT Biological Engineering
(Fall 2019)

Part of the teaching team for the introductory graduate course 20.420 - Principles of Molecular Bioengineering, taught by Profs. Ernest Fraenkel and Alan Jasanoff. Topics include reaction kinetics modeling, protein thermodynamics and computational prediction, and systems-level models of metabolism. Assisted with designing and grading assignments and exams, providing review lecture sessions, and providing one-on-one tutoring for 40 students in the Biological Engineering, Chemical Engineering, and Health Sciences and Technology graduate programs. Also conducted a programming bootcamp session, with materials available here.

Teaching Assistant – Uniandes Biomedical Engineering
(Spring, Fall 2016)

Part of the teaching team for the second-year undergraduate courses Quantitative Human Physiology I and II, taught by Prof. Juan M. Cordovez. Topics include human anatomy and systems physiology as well as mathematical modeling of physiological processes. Assisted with designing and grading assignments and exams, providing review lecture sessions, and providing one-on-one tutoring for 100 students in the Biomedical Engineering undergraduate program. Also taught two main lectures during professor absences.

Teaching Assistant – Uniandes Student Affairs
(Fall 2015)

Part of the teaching team for Social Practice, an undergraduate course and outreach program taught by instructors David Parga and María del Pilar Pérez. Assisted with training, guiding, and evaluating 60 Uniandes undergraduate students of all majors and years serving as academic tutors for low-income high school students in Bogotá, Colombia.

Teaching Assistant – Uniandes Biological Sciences
(Spring 2015)

Part of the teaching team for the Parasitology Laboratory course, a second year undergraduate course taught by Prof. Camila González and instructor Laura Tamayo. Topics included anatomy, physiology, and cell biology of diverse human and animal parasites and tropical diseases, from roundworms to viral fevers. Assisted by providing review sessions and tutoring, helping develop and grade assignments, and preparing demonstrative microscopy slides.

You are welcome to

• hack my coding projects on GitHub
• engage with my science views on Mastodon, Bluesky, Threads, or Twitter.
• follow my research on LinkedIn and ResearchGate
• view my published work on Google Scholar and ORCiD
• download my CV
• reach out to pablocarderam@gmail.com

I love collaborating on projects or just hearing questions, suggestions, or thoughts!

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